Angen Biotech Co.,Ltd

Hipure DNA Micro Column

G-DNA Micro Column provides for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples. DNA RecoveryUp to 5 & 181g total DNA is eluted into =10 & 181l (6 & 181l minimum) DNA Elution Buffer or water. Human whole blood will typically yield 1.5-3.5 & 181g DNA per 50 & 181l blood sampled. Mammalian tissues already homogenized yield: 1-3 & 181g DNA per mg skeletal, heart, and brain tissues and 3-5 & 181g DNA per mg liver, kidney and lung tissues.Equipmentmicrocentrifuge, vortexDNA Size LimitsCapable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.Sample SourcesWhole blood, plasma, or serum from humans, mice, rats, etc. Also, cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized can also be processed.DNA PurityHigh-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8Product Detergent Tolerance=5 Percent Triton X-100, =5 Percent Tween-20, =5 Percent Sarkosyl, =0.1 Percent SDS.Workflow OverviewUnique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.

View All Products posted by Angen Biotech Co.,Ltd