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The AG 96 Well DNA Binding Column is designed for high-throughput purification of DNA from 1-5 ml of cultured bacterial cells per well using an efficient 96 well filter plate and 96 well binding plate system. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.AdvantagesPurify 96 wells of pure genomic DNA in 45 minutes!Sample Volume: up to 200 & 181l of whole blood, plasma, serum, body fluids and up to 5 x 106 lymphocytes or cultured cells per wellYield: up to 6 µg of genomic DNA from 200 & 181l of whole blood per well Format: 96 Well Binding PlatesElution Volume: 200 ~ 400 & 181lCentrifuge or vacuum manifold protocolStorage: dry at room temperature (15-25& 186C)
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