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Important notes1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.2.washing buffer will Crystal`lization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(& 215n& 2155).5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.6.The subst`rate evade the light preservation.7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.8.All samples, washing buffer and each kind of reject should according to infective material process.9.Do not mix reagents with those from other lots.Calculate Assay range4& 181g/L -200& 181g/LStorage and validity1.Storage: 2-8?.2.validity: six months.
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