www.glorybioscience.comExport DepartmentAngelia ChangPhone: +86 - 0571 - 88554421/+86 - 15088872987Fax: +86 - 0571 - 88554421
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The kit assay Rat irisin level in the sample, use Purified Rat irisin to coat microtiter plate wells, make solid-phase antibody, then add irisin to wells, Combine irisin antibody with HRP labeled to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution,TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of irisin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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