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The kit employs a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human irisin in samples. Add irisin to monoclonal antibody Enzyme well which is pre-coated with Human irisin monoclonal antibody and incubation After that, add irisin antibodies labeled with biotin and combined with Streptavidin-HRP to form immune complex Again carry out incubation and washing to remove the uncombined enzyme. Then the color of the liquid will turn to blue after adding Chromogen Solution A, B and will finally turn into yellow with the effect of acid. The chroma of color and the concentration of the Human irisin of sample were positively correlated.
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