http://www.creative-diagnostics.comExport DepartmentLily GreenPhone: +1 - 631 - 624-4882/+1 - Fax: +1 - 631 - 614-7828
45-16 Ramsey Road ShirleyNew York, New YorkUnited States, 11967Web Page Link: http://www.toboc.com/creativediagnostics
TheELISA kit is a solid phase sandwich ELISA (Enzyme-Linked ImmunosorbentAssay). It utilizes a monoclonal antibody specific for EPCAM coated on a96-well plate. Standards and samples are added to the wells, and any EPCAMpresent binds to the immobilized antibody. The wells are washed and abiotinylated rabbit anti-EPCAM monoclonal antibody is then added, producingan antibody-antigen-antibody “sandwich”. To produce color in proportion tothe amount of EPCAM pre-sent in the sample streptavidin-HRP and TMB subst`ratesolution are loaded. The absorbances of the microwell are read at 450nm.....
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